|
|
| Poyer, Salome; Loutelier-bourhis, Corinne; Coadou, Gael; Mondeguer, Florence; Enche, Julien; Bossee, Anne; Hess, Philipp; Afonso, Carlos. |
| The aim of this work was to develop a reliable and efficient analytical method to characterise and differentiate saxitoxin analogues (STX), including sulphated (gonyautoxins, GTX) and non-sulphated analogues. For this purpose, hydrophilic interaction liquid chromatography (HILIC) was used to separate sulphated analogues. We also resorted to ion mobility spectrometry to differentiate the STX analogues because this technique adds a new dimension of separation based on ion gas phase conformation. Positive and negative ionisation modes were used for gonyautoxins while positive ionisation mode was used for non-sulphated analogues. Subsequently, the coupling of these three complementary techniques, HILIC-IM-MS, permitted the separation and identification of STX... |
| Tipo: Text |
Palavras-chave: Saxitoxin; Ion mobility-mass spectrometry; Isomers; Multidimensional separation; HILIC. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00249/36067/34924.pdf |
| |
